WebActin-Tracker Green-488探针为荧光染料Alexa Fluor 488标记的鬼笔环肽(phalloidin, 又称鬼笔毒素或毒蕈肽)，即phalloidin- Alexa Fluor 488，最大激发波长为495nm，最大发射波长为518nm。Actin-Tracker Green-488的荧光光谱和FITC比较接近，可以用FITC的检测条件进行 … WebJan 17, 2024 · Phalloidin staining and confocal microscopy Cells were plated on confocal dishes coated with poly-L-lysine. After incubation at 37 °C for 24 h, cells were fixed with 4% paraformaldehyde for 30 min, and were stained with 5 μg/mL Alexa Fluor 488-phalloidin (Invitrogen) to reveal filamentous actin (F-actin) in PBS for 40 min at 37 °C.

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WebPhalloidin, Alexa Fluor ™ Plus 647 Phalloidin, and Alexa Fluor Plus 750 Phalloidin. Note: For a complete list of fluorescent or biotinylated phalloidin conjugates, see Table 1 on page 5. Contents and storage Contents Cat. No. Amount[1] Storage[2] Fluorescent phalloidin See Table 1 on page 5. 1 vial of lyophilized solids (300 units/assay[3 ... WebProduct overview. Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be … prima information solutions inc

Fluorescent Phalloidin: A Practical Stain for Visualizing Actin ...

WebAlexa Fluor® 488. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing ... WebPhalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized. WebAdd 1 µL of AF488 Phalloidin solution to 1 mL of PBS with 1% BSA. N o te The stock solution of phalloidin conjugate should be aliquoted and stored at -20 °C. protected from light. N o te Different cell types might be stained differently. The concentration of phalloidin conjugate working solution should be prepared accordingly. platinum vs gold price per ounce