S5a protein

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August undefined, 2024

WebThe sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions 27 and 54, respectively. The original pACT-derived cDNA, clone 14A, that was isolated based on the interaction of its encoded protein with Id1 begins at codon 34. The entire reading frame encodes a protein of 377 amino acids. WebDec 15, 2009 · Decreased 19S-S5a protein levels and MGO-modified 20S-β2 proteasome subunit in GLO1-knockdown (GL01-KD) and STZ diabetic mice. Kidney lysate protein (40 …

Recombinant Human His6-S5a/Angiocidin Protein, CF

WebApr 23, 2009 · S5a, fusion proteins containing S5a's UIMs or HHR23A was added to ubiquitination mixture from the outset and GST was added as a control of GST-UIM. For the reaction with GST and GST-UIM, a different batch of enzymes (E1, E2 and CHIP) from other reactions was used. WebSep 24, 1999 · S5a is known as a multiubiquitin chain-binding protein. Since both hHR23 proteins have highly conserved Ub-related sequences on NH 2 termini ( 1, 2 ), we speculated that these sequences are involved in the interaction with S5a. Table I. Protein-protein interactions assessed by the yeast two-hybrid system ghost products protein

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Web55 minutes ago · For fusion protein production, the DNA was transformed into BL21(DE3) competent cells for high protein expression and induced mid-log phase with 0.4 mM IPTG 16–20 h at 22 °C. WebJan 1, 2005 · The S5a protein was immunoprecipitated from 3 mg of cell-lysate protein, and the immunoprecipitates were analysed for the presence of VCP, SAKS1, S4 and S5a by immunoblotting. Figures - uploaded ... WebMay 8, 2009 · S5a/Rpn10 is a ubiquitin (Ub)-binding protein that is a subunit of the 26S proteasome but also exists free in the cytosol. It binds poly-Ub chains through its two Ub-interacting motifs (UIMs). We discovered that, unlike typical substrates of Ub ligases … National Center for Biotechnology Information ghost profile breakpoint

DNA-repair protein hHR23a alters its protein structure upon …

WebApr 5, 2024 · As expected, the protein expression of p-ATM was significantly down-regulated by the treatment with KU55933, as was the level of its downstream molecule p-Chk2 (Figure 5e). More importantly, the mRNA levels of SASP-related factors including IL-1α, IL-1β, IL-6, IL-8, CXCL10, and MMP3 were markedly suppressed by KU55933 treatment ( Figure 5f ... WebApr 11, 2024 · Fabp5 is one kind of fatty acid binding protein that responds to chemical-induced skin inflammation and tumorigenesis [32]. Cd74, the macrophage migration inhibitory ... (Fig. S5A), therefore we further investigated the heterogeneity and transcriptional profiles of PBC in the AEW model. We re-analyzed PBC and found that they … front loader washer detergentWebC5a protein is composed of alpha and beta polypeptide chains, which are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin, which has potent … front loader washer dryer pair

"WebIdentification of the Ub-like sequence as the S5a-interacting domain of hHR23B raised the possibility that the S5a protein binds to any proteins which are conjugated with ubiquitin. To examine whether this is the case, we performed competition experiments using either free ubiquitin itself or the NH 2-terminal Ub-like domain of hHR23B. " - S5a protein

S5a protein

S5a promotes protein degradation by blocking synthesis of …

WebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. … WebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein.

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WebOct 14, 2013 · S5a is required for the degradation of p53 but not its E3 ligase Mdm2. Additional work is required to completely define the pathways of proteasomal recognition … WebDownload scientific diagram DNA and protein sequence of S5a. The sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions …

WebSep 13, 2006 · The results suggest that the protein folding reaction is linked to topology only in the sense that the native-state topology determines the repertoire of accessible … WebApr 11, 2003 · Total Structure Weight: 9.52 kDa Atom Count: 668 Modelled Residue Count: 85 Deposited Residue Count: 85 Unique protein chains: 1 Display Files Download Files 1P1A NMR structure of ubiquitin-like domain of hHR23B PDB DOI: 10.2210/pdb1P1A/pdb Classification: DNA BINDING PROTEIN Organism (s): Homo sapiens Expression System: …

Web26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein : Clone: S5a-18 : Host: Mouse : Isotype: IgG1 : Immunogen: Recombinant full-length human Rpn10 protein. UniProt ID: P55036 : Gene/Protein Identifier: PSMD4 (gene name) Source: Purified from hybridoma tissue culture supernatant. Species ... WebMar 21, 2024 · GeneCards Summary for S100A5 Gene. S100A5 (S100 Calcium Binding Protein A5) is a Protein Coding gene. Diseases associated with S100A5 include …

WebParkin is a multidomain E3 ligase associated with autosomal recessive Parkinson disease. The N-terminal ubiquitin-like domain (Ubld) of parkin functions with the S5a proteasomal subunit, positioning substrate proteins for degradation. In addition the parkin Ubld recruits the endocytotic protein Eps15, allowing the E3 ligase to ubiquinate Eps15 distal from its …

WebS5A antibody; S5a/antisecretory factor protein antibody; see all. Images. Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab18512) Crude Drosophila extract. Secondary antibody: rabbit polyclonal 1:5000. Please note: Drosophila S5a has an actual mass of 42.6 kDa, however, on the gel it runs at an apparent 54 kDa band. front loader washer pedestal geWebSep 7, 2009 · Particular attention was paid to the 19S-RP protein, S5a/PSMD4, and the 20S-CP protein, α-5/PSMA5, expected to be involved in the exaggerated proteasome activity in cancer (Murata et al., 2009). ghost profile wildlandsWebJul 27, 2007 · Monoubiquitinated Pax3 was shuttled to the intrinsic proteasomal protein S5a by interacting specifically with the ubiquitin-binding protein Rad23B. Functionally, sustained expression of Pax3 proteins inhibited myogenic differentiation, demonstrating that Pax3 degradation is an essential step for the progression of the myogenic program. front loader washer leakingWebOct 13, 2003 · The Binding Surfaces of S5a and the UBA Domains Overlap. We used an S5a construct spanning residues M196 to D307 to study the effect of S5a-binding on hHR23a protein structure. S5a (196–307) contains the two ubiquitin-binding domains and was previously shown to bind hHR23a with equal affinity to full-length S5a . ghost program for computerWebNational Center for Biotechnology Information front loader washer not tumblingWebJul 7, 2013 · S5a/Rpn10 drives dendrite growth and elaboration in primary neurons and in the rodent brain in vivo We characterized the function of the key proteasomal lid-associated protein S5a/Rpn10 in the mammalian brain, utilizing granule neurons of the developing rat cerebellar cortex. ghost profitWebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ... ghost program free download